Composite

Part:BBa_K519003:Design

Designed by: Chifuku Mita   Group: iGEM11_Tokyo-NoKoGen   (2011-09-28)

Inducible BMC shell protein generator with GFP fused pduP1~18 tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2821
    Illegal NheI site found at 2844
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1755
    Illegal BamHI site found at 3449
    Illegal XhoI site found at 3350
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2539
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Citrobacter freundii

Citrobacter freundii is not infectious.

References

"Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement" Joshua B. Parsons et al. Molecular Cell 38, 305.315, April 23, 2010

"Short N-terminal sequence package proteins into bacterial microcompartments", Chenguang Fan et al., PNAS, April 20 1010, vol.107 no.16 7509-7514